Standardisation of concentration measurements of extracellular vesicles for medical diagnoses

METVES II overview


Extracellular vesicles (EVs) are cell-derived particles in body fluids, which have excellent potential as next generation biomarkers for the early diagnosis of common diseases, such as cancer and thrombosis. This project aims to tap into the clinical potential of EVs by developing traceable measurements of number concentration, size distribution, refractive index (RI) and fluorescence intensity of cell-specific EVs in human blood and urine.

To realise our aims, we will develop synthetic reference materials with physical properties resembling EVs (work package 1), instrumentation and procedures to standardise EV measurements in clinical laboratories (work package 2), and ready-to-use biological test samples (work package 3). These will then be evaluated in an inter-laboratory comparison study across a range (>20) of standard flow cytometers in clinical labs (work package 4).


WP overview METVES II
Overview of the work packages (WPs) of METVES II. EV: extracellular vesicles; RI: refractive index.